Karen Abramson, MPH



Established in 1996, the Duke Molecular Physiology Institute (DMPI) Molecular Genomics Core (MGC) is a state of the art facility that offers a variety of experimental platforms to facilitate genomics research. 

Applications include 10x Genomics NGS library preps for both single cell and gDNA experiments, Illumina DNA methylation arrays, Qiagen pyrosequencing for targeted DNA methylation assays, Illumina SNP genotyping and copy number microarrays, and Taqman targeted SNP genotyping. 

The MGC staff offer decades of experience with genetic, genomic and epigenomic study design and technology, and work closely with researchers to customize experiments to meet their needs.

Services and Technologies

10x Genomics NGS Library Prep

    a) 10x Genomics Single-cell Solutions

  • Gene expression
  • V(D)J profiling in T and B cells
  • ATAC-seq
  • Copy number variation
  • Cell surface protein expression

    b) 10x Genomics whole genome “linked read” libraries

DNA Methylation

  • Genome-wide:  Illumina methylationEPIC Arrays
  • Targeted:  Qiagen PyroMark CpG Assays

SNP Genotyping and Copy Number Variation

  • Genome-wide:  Illumina microarrays
  • Targeted:  Illumina microarrays and Life Technologies Taqman Assays

Sample Quantitation and Quality Assessment


10X Genomics Chromium Controller

  • Unique reagent delivery system that partitions individual cells or HMW DNA molecules into 100,000s to 1,000,000s of uniquely addressable partitions

Illumina iScan System and Autoloader 2

  • High-precision, high-throughput array scanning for Illumina’s expansive portfolio of genetic analysis assays

Qiagen PyroMark Q24 Advanced

  • Pyrosequencing for quantitative methylation analysis at consecutive CpG or CpN sites

Applied Biosystems ViiA 7 Real-Time PCR System and Twister II

Sample Quantitation and Quality Assessment Instruments

  • Agilent 4200 TapeStation
  • Thermo Scientific Qubit 3.0 Fluorometer
  • Tecan Infinite M200Pro Scanner
  • Nexcelom Cellometer K2 Fluorescent Viability Cell Counter

Sample Handling Instruments

  • Eppendorf epMotion 5075 Robotic Liquid Handling System
  • Tecan Freedom Evo 150 Robotic Liquid Handling System
  • BioMicroLab XL-20 Tube Sorter


Estimate and Service requests can be submitted through CoreResearch@Duke. Our core is listed as D-0020 DMPI: Molecular Genomics. You can find procedure guides and sample submission forms on our website.   

If you have any questions or would like to schedule a consultation, please contact us


Fees are listed in CoreResearch@Duke, but for a full and accurate estimate for your project please submit an estimate request. DCI members are charged a reduced labor rate.